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Neuroscience
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主培養海馬ニューロンを用いた軸索初期セグメントの組み立て研究
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
Please note that all translations are automatically generated.
Click here for the English version.
主培養海馬ニューロンを用いた軸索初期セグメントの組み立て研究
DOI:
10.3791/61411-v
•
06:53 min
•
February 12, 2021
•
Rui Yang
2,3
,
Vann Bennett
3
1
School of Basic Medical Sciences
,
Xi’an Jiaotong University
,
2
Department of Cell Biology
,
Duke University
,
3
Department of Biochemistry
,
Duke University
Chapters
00:04
Introduction
00:24
Culture Hippocampal Neurons
02:37
Disruption of AIS by Knockout of Ankyrin-G at an Early Stage of Neuron Development
04:17
Quantification of Axon Initial Segment
04:58
Results: A Full Rescue of AIS by 480kDa AnkG in
ANK3
-E22/23-flox Neurons Transfected with Cre
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、巨大なアンキリンGがないために組み立て済みのAISを欠く海馬ニューロンの軸索初期セグメント(AIS)の組み立てと構造を定量的に研究するプロトコルについて説明した。
Tags
Primary Cultured Hippocampal Neurons
Axon Initial Segments
Ankyrin-G
CRE BFP DNA
Ankyrin-G GFP DNA
Transfection
Dissection
Trypsin Digestion
Trituration
Neuronal Culture
Glial Cell Feeder Dishes
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