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Forudsigelse af amputation ved hjælp af lokale cirkulerende mononukleare stamceller i angioplastikbehandlede patienter med kritisk limbiskæmi
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JoVE Journal Medicine
Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia

Forudsigelse af amputation ved hjælp af lokale cirkulerende mononukleare stamceller i angioplastikbehandlede patienter med kritisk limbiskæmi

DOI:
10.3791/61503-v

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07:25 min

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September 22, 2020

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, , , , , , , , , , , , ,
1Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research,Centro Médico Nacional 20 de Noviembre, ISSSTE, 2Vascular Surgery and Angiology Department,Centro Médico Nacional 20 de Noviembre, ISSSTE, 3Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research,Centro Médico Nacional 20 de Noviembre, ISSSTE

Chapters

  • 00:04Introduction
  • 01:33Flow-Mediated Dilation (FMD) Evaluation
  • 02:11Lower Limb Vascular Blood Evaluation, Blood Sampling, and Balloon Angioplasty
  • 04:07Circulating Mononuclear Progenitor Cell (MPC) Isolation and Antibody Staining
  • 05:21MPC Quantification
  • 06:10Results: Representative Relationship Between MPC and Hemodynamic Indicators and Lower Limb Amputation Prognosis After Angioplasty
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

Amputation af underekstremiteterne kan forekomme selv efter angioplastik af blokerede beholdere i Critical Limb Ischemia (CLI). Mononukleare stamceller afspejler vaskulær reparation. Denne protokol beskriver kvantificeringen af MPC'er fra cirkulation tæt på angioplastik, og dens forhold til endotel dysfunktion og forudsigelse af underekstremitet amputation.

Tags

– Angioplasty- Critical Limb Ischemia- Limb Amputation- Mononuclear Progenitor Cells (MPCs)- Endothelial Dysfunction- Vascular Events- Ischemic Heart Disease- Lower Limb Ischemia- Risk Stratification- Rutherford Classification- Brachial Artery Diameter- Flow-mediated Dilation (FMD)- Vascular Obstruction- Angioplasty Balloon Catheter

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