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1Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research,Centro Médico Nacional 20 de Noviembre, ISSSTE, 2Vascular Surgery and Angiology Department,Centro Médico Nacional 20 de Noviembre, ISSSTE, 3Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research,Centro Médico Nacional 20 de Noviembre, ISSSTE
Chapters
- 00:04Introduction
- 01:33Flow-Mediated Dilation (FMD) Evaluation
- 02:11Lower Limb Vascular Blood Evaluation, Blood Sampling, and Balloon Angioplasty
- 04:07Circulating Mononuclear Progenitor Cell (MPC) Isolation and Antibody Staining
- 05:21MPC Quantification
- 06:10Results: Representative Relationship Between MPC and Hemodynamic Indicators and Lower Limb Amputation Prognosis After Angioplasty
- 07:04Conclusion
A amputação de membros inferiores pode ocorrer mesmo após a angioplastia de vasos obstruídos em Isquemia de Membros Críticos (CLI). Células Progenitoras Mononucleares (MPCs) refletem o reparo vascular. O presente protocolo descreve a quantificação de CMS de circulação próxima à angioplastia, e sua relação com disfunção endotelial e previsão de amputação de membros inferiores.
