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Journal / Biology / GFP 발현 골세포의 쥐 두개골 분획을 통한 1차 골세포…
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GFP 발현 골세포의 쥐 두개골 분획을 통한 1차 골세포 획득

Article DOI: 10.3791/61513-v • 07:22 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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이 프로토콜은 형광 활성화 세포 분류(FACS)를 위한 골세포 준비 외에도 신생아 dmp1-topaz 마우스 두개골의 해부와 세포 소화 및 분획을 통해 녹색 형광 단백질을 발현하는 골세포의 분리를 설명합니다.

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철회 문제 160 원발성 골세포 Dmp1 SOST GFP 분획 FACS
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