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Journal / Biology / GFP eksprese eden osteositlerin murin kalvarial…
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GFP eksprese eden osteositlerin murin kalvarial fraksiyonasyonu yoluyla primer osteositlerin elde edilmesi
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GFP eksprese eden osteositlerin murin kalvarial fraksiyonasyonu yoluyla primer osteositlerin elde edilmesi

Article DOI: 10.3791/61513-v • 07:22 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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Bu protokol, floresan aktive hücre sıralama (FACS) için osteosit hazırlığına ek olarak, yenidoğan dmp1-topaz fare kalvarisinin diseksiyonunu ve yeşil floresan proteinini hücre sindirimi ve fraksiyonasyonu yoluyla eksprese eden osteositlerin izolasyonunu açıklar.

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Retraksiyon Sayı 160 Primer osteosit Dmp1 SOST GFP Fraksiyonasyon FACS
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