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1Department of Medicine,University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program,University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology,Rensselaer Polytechnic Institute, 4Department of Health Sciences,Curtin University, 5Department of Medicine,Denver Health Medical Center
Chapters
- 00:04Introduction
- 00:55Polyacrylamide Gel Electrophoresis of Isolated and Purified Glycosaminoglycans
- 02:48Silver Staining Protocol
- 03:55Results: Sensitivity and Specificity of Method of Detection of GAGs
- 05:27Conclusion
This report describes techniques to isolate and purify sulfated glycosaminoglycans (GAGs) from biological samples and a polyacrylamide gel electrophoresis approach to approximate their size. GAGs contribute to tissue structure and influence signaling processes via electrostatic interaction with proteins. GAG polymer length contributes to their binding affinity for cognate ligands.
