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Laser-Capture Microdissection RNA-sekventering for rumlige og tidsmæssige vævsspecifikke genekspressionsanalyser i planter
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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-sekventering for rumlige og tidsmæssige vævsspecifikke genekspressionsanalyser i planter

English

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7,662 Views

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08:33 min

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August 05, 2020

DOI:

10.3791/61517-v

DOI:
10.3791/61517-v

•

08:33 min
August 05, 2020

•

6 Views
, , , , ,
1Department of Animal, Plant and Soil Science, AgriBio Building,La Trobe University, 2Australian Research Council Centre of Excellence in Plant Energy Biology,La Trobe University, 3Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building,La Trobe University

Summary

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Præsenteret her er en protokol for laser-capture microdissection (LCM) af plantevæv. LCM er en mikroskopisk teknik til isolering af vævsområder på en forureningsfri måde. Proceduren omfatter vævsfiksering, paraffinindlejring, sektionsindtrækning, LCM- og RNA-ekstraktion. RNA anvendes i den downstream-vævsspecifikke, tidsmæssigt løste analyse af transskriptomer.

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Cite this Article

Liew, L. C., Wang, Y., Peirats-Llobet, M., Berkowitz, O., Whelan, J., Lewsey, M. G. Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants. J. Vis. Exp. (162), e61517, doi:10.3791/61517 (2020).

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