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Dynamisk måling og billeddannelse af kapillærer, arterioler og pericytter i musehjerte
JoVE Journal
Medicine
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JoVE Journal Medicine
Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart
DOI:

07:16 min

July 29, 2020

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Chapters

  • 00:04Introduction
  • 00:41Cannula Preparation
  • 01:39Mouse Heart Extraction
  • 02:33Septal Artery Preparation and Cannulation
  • 03:47Preparation Stabilization
  • 04:38Fluorescence-Tagged Wheat-Germ Agglutinin (WGA) Loading and Confocal Arteriole and Capillary Imaging
  • 05:42Results: Representative Capillary and Arteriole Imaging and Luminal Pressure and Atrial Diameter Assessment
  • 06:42Conclusion

Summary

Automatic Translation

Præsenteret her er en protokol til at studere koronar mikrocirkulation i levende murin hjertevæv ved ex vivo overvågning af arteriel perfusion tryk og flow, der opretholder trykket, samt vaskulære trækomponenter, herunder kapillær senge og pericytes, som septal arterie er cannulated og tryk.

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