A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 00:41Cannula Preparation
- 01:39Mouse Heart Extraction
- 02:33Septal Artery Preparation and Cannulation
- 03:47Preparation Stabilization
- 04:38Fluorescence-Tagged Wheat-Germ Agglutinin (WGA) Loading and Confocal Arteriole and Capillary Imaging
- 05:42Results: Representative Capillary and Arteriole Imaging and Luminal Pressure and Atrial Diameter Assessment
- 06:42Conclusion
Præsenteret her er en protokol til at studere koronar mikrocirkulation i levende murin hjertevæv ved ex vivo overvågning af arteriel perfusion tryk og flow, der opretholder trykket, samt vaskulære trækomponenter, herunder kapillær senge og pericytes, som septal arterie er cannulated og tryk.
