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Kvantificering af cirkulerende gris-specifikke DNA i blodet af en Xenotransplantation Model
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Kvantificering af cirkulerende gris-specifikke DNA i blodet af en Xenotransplantation Model

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020
September 22nd, 2020 •
*1,2, *1,3, 1, 1, 4, 1,5, 6, 7, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally

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I denne protokol blev der designet svinespecifikke primere, der blev fremstillet plasmiderholdige svinespecifikke DNA-fragmenter, og der blev etableret standardkurver for kvantitet. Ved hjælp af artsspecifikke primere blev cpsDNA kvantificeret af qPCR i svine-til-mus-celletransplantationsmodeller og svin-til-abe-arteriepulptransplantationsmodeller.

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Biologi specifikke primere cirkulerende gris-specifikke DNA qPCR xenotransplantation biomarkør immunafstødning
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