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1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center,Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology,Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, 5Department of Life Sciences,University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery,Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital
Chapters
- 00:04Introduction
- 00:52Isolating Genomic DNA
- 02:20Verification of Primer Specificity
- 03:42Standard Curve of cpsDNA
- 04:45Quantitation of Circulating Pig-specific DNA
- 05:55Results: PCR Validation of Porcine Specific Primers
- 06:51Conclusion
I denne protokollen ble svinespesifikke primere designet, plasmider-inneholdende svinspesifikke DNA-fragmenter ble konstruert, og standardkurver for kvantitet ble etablert. Ved hjelp av artsspesifikke primere ble cpsDNA kvantifisert av qPCR i celletransplantasjonsmodeller for gris-til-mus og gris-til-ape arterie patch transplantasjon modeller.
