Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Quantifizierung der zirkulierenden…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Quantifizierung der zirkulierenden schweinespezifischen DNA im Blut eines Xenotransplantationsmodells
Gastrointestinal Motility Monitor (GIMM)
Journal (Biology)
Gastrointestinal Motility Monitor (GIMM)
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
Journal (Biology)
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
 
Click here for the English version

Quantifizierung der zirkulierenden schweinespezifischen DNA im Blut eines Xenotransplantationsmodells

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020
September 22nd, 2020 •
*1,2, *1,3, 1, 1, 4, 1,5, 6, 7, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

In diesem Protokoll wurden schweinespezifische Primer entwickelt, Plasmiden enthaltende Porenspezifische DNA-Fragmente konstruiert und Standardkurven für die Quantifizierung festgelegt. Anhand artspezifischer Primer wurde cpsDNA durch qPCR in Schweine-zu-Maus-Zelltransplantationsmodellen und Schweine-zu-Affen-Arterien-Patch-Transplantationsmodellen quantifiziert.

Tags

Biologie Ausgabe 163 spezifische Primer zirkulierende schweinespezifische DNA qPCR Xenotransplantation Biomarker Immunabstoßung
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter