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Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging
JoVE Journal
Biology
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JoVE Journal Biology
Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging

Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging

DOI:
10.3791/61604-v

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07:44 min

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July 28, 2020

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,
1Center for Vascular Research,Institute for Basic Science

Chapters

  • 00:05Introduction
  • 00:57Isolation of Deep Ectoderm Cells
  • 03:09Generation of Mucociliary Epithelial Organoids
  • 04:29High-resolution Live Imaging of Developing Organoids
  • 05:23Results: Imaging of Developing Organoids
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a simple protocol to develop mucociliary epithelial organoids from deep ectoderm cells isolated from Xenopus laevis embryos. The multipotent progenitors regenerate epithelial goblet cell precursors and allow live tracking of the initiation and progression of the cell transitions on the surface of organoids.

Tags

Keywords: Mucociliary EpitheliumXenopus Embryonic CellsOrganoid GenerationLive Cell ImagingCell TransitionsRapid ProtocolScalableReproducibleEmbryo CultureAnimal Cap ExcisionDeep Ectoderm Separation

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