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Neuroscience
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无转基因生成血液衍生神经元细胞
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
GM-Free Generation of Blood-Derived Neuronal Cells
Please note that all translations are automatically generated.
Click here for the English version.
无转基因生成血液衍生神经元细胞
DOI:
10.3791/61634-v
•
08:11 min
•
February 13, 2021
•
Zorica A. Becker-Kojić
,
Anne-Kathrin Schott
,
Ivan Zipančić
,
Vicente Hernández-Rabaza
1
ACA CELL Biotech GmbH
,
2
Departmento Ciencias Biomedicas
,
Universidad Cardenal Herrera-CEU
Chapters
00:04
Introduction
00:47
Isolation of Human Peripheral Blood Mononuclear Cells (PBMNCs
01:25
Activation of Human GPI-Anchored Glycoprotein by Antibody Crosslinking on the Surface of PBMNCs
02:21
Sorting of Newly Generated Dedifferentiated Cells
03:33
Preparing Cell Culture Dishes for Neuronal Differentiation of Newly Generated Stem Cells
04:10
Culturing of Neuronal Differentiated Blood Cells
04:44
Immunofluorescence Microscopy Analysis of Blood-Derived Neural Cells
06:08
Results: Morphology, Re-differentiation and Immunophenotyping of Generated Neuronal Cells
07:41
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种无转基因(无转基因)的方法,从重新编程的周围血细胞中获取具有神经元表型的细胞。激活与新型人类GPI相关蛋白质相关的信号通路,揭示了获得人类多能干细胞的高效无转基因方法。
Tags
Blood-derived Neuronal Cells
GM-free
Membrane To Nucleus Signaling
Non-teratogenic
Regenerative Medicine
PBMNCs
Density Gradient Media
Antibody Cross-linking
GPI-linked Membrane Protein
Iscove’s Modified Dulbecco’s Medium
CD-45 Positive Nano-sized Magnetic Beads
Cell Separation
Cell Culture
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