Journal
/
Cancer Research
/
使用有机体片 3D 模型、机器学习和共声断层扫描量化脑转移肿瘤微环境
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Quantifying the Brain Metastatic Tumor Micro-Environment using an Organ-On-A Chip 3D Model, Machine Learning, and Confocal Tomography
Please note that all translations are automatically generated. Click here for the English version.

使用有机体片 3D 模型、机器学习和共声断层扫描量化脑转移肿瘤微环境

DOI:
10.3791/61654-v

•

09:53 min

•

August 16, 2020

•

, , ,
1Department of Internal Medicine,University of Michigan Ann Arbor, 2Rogel Cancer Center,University of Michigan Ann Arbor, 3Department of Neurosurgery,University of Michigan Ann Arbor, 4Department of Cell and Developmental Biology,University of Michigan Ann Arbor

Chapters

  • 00:04Introduciton
  • 00:49Form and Assemble the Microfluidic BBN Device
  • 03:24Cancer Cell Seeding
  • 04:023D Model Imaging Data Conversion
  • 05:06Plane Fitting
  • 06:04Individual Cancer Cell Analysis
  • 07:05Results: Representative Brain Metastatic Tumor Micro-Environment Analyses
  • 08:56Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一个协议，用于准备和培养血脑屏障转移肿瘤微环境，然后使用共体成像和人工智能（机器学习）量化其状态。

Tags

Confocal TomographyMachine LearningOrgan-on-a-chipBrain MetastasisTumor MicroenvironmentCell BehaviorDiagnostic ToolMicrofluidic DeviceCollagenAstrocyteEndothelial CellsCell Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克

肝大肠癌转移的高级动物模型：成像技术和转移性克隆性质

肿瘤组织中高通量芯片测序的染色质状态全基因组图谱集成平台

利用小鼠脑肿瘤球的原发染色质沉淀

超声引导组织定向细胞植入术在生物学相关转移性肿瘤移植中的应用

活体细胞成像技术研究胶质瘤脑干细胞的迁移和侵袭

数字聚合酶链反应法测定零星家族性腺瘤性息肉病患者的基因变异

肿瘤治疗胶质母细胞瘤的临床应用

三维肿瘤球体的纵向形态和生理监测

通过高分辨率共聚焦显微镜对3D肿瘤球体入侵的影响表征

Read Article