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农业细菌
-中生菌遗传转化、转基因生产及其在水稻雄性生殖发育研究中的应用
JoVE Journal
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JoVE Journal
Biology
Agrobacterium
-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
Please note that all translations are automatically generated.
Click here for the English version.
农业细菌
-中生菌遗传转化、转基因生产及其在水稻雄性生殖发育研究中的应用
DOI:
10.3791/61665-v
•
07:43 min
•
October 06, 2020
•
Dawei Xu*
1,2
,
Palash Chandra Mondol*
1
,
Muhammad Uzair
,
Matthew R. Tucker
,
Dabing Zhang
3
1
Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology
,
Shanghai Jiao Tong University
,
2
College of Agronomy
,
Anhui Agricultural University, Heifei, China
,
3
School of Agriculture, Food and Wine
,
University of Adelaide
Chapters
00:04
Introduction
00:54
Rice Genetic Transformation and Plant Tissue Culture
04:00
Observation of the Basic Phenotype of the Mutant
05:14
Results: Targeted Mutagenesis of the
OsABCG15
07:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项工作描述了使用CRISPR-Cas9基因组编辑技术敲除内源
基因OsABCG15,
然后修改的农业细菌
中
培养转化方案,在水稻中产生稳定的雄性-无菌线。
Tags
Agrobacterium-mediated Transformation
Transgenic Production
Rice
Male Reproductive Development
Young Inflorescences
Callus Production
Gene Transformation
Regeneration
Video Demonstration
Meiosis Stage
NBD2 Medium
EB Plate
YEB Medium
AAM AS Medium
Embryogenic Calli
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