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农业细菌-中生菌遗传转化、转基因生产及其在水稻雄性生殖发育研究中的应用
JoVE Journal
Biology
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JoVE Journal Biology
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
DOI:

07:43 min

October 06, 2020

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Chapters

  • 00:04Introduction
  • 00:54Rice Genetic Transformation and Plant Tissue Culture
  • 04:00Observation of the Basic Phenotype of the Mutant
  • 05:14Results: Targeted Mutagenesis of the OsABCG15
  • 07:16Conclusion

Summary

Automatic Translation

这项工作描述了使用CRISPR-Cas9基因组编辑技术敲除内源 基因OsABCG15, 然后修改的农业细菌 培养转化方案,在水稻中产生稳定的雄性-无菌线。

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