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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
JoVE Journal
Biology
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JoVE Journal Biology
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
DOI:

07:43 min

October 06, 2020

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Chapters

  • 00:04Introduction
  • 00:54Rice Genetic Transformation and Plant Tissue Culture
  • 04:00Observation of the Basic Phenotype of the Mutant
  • 05:14Results: Targeted Mutagenesis of the OsABCG15
  • 07:16Conclusion

Summary

Automatic Translation

Diese Arbeit beschreibt den Einsatz der CRISPR-Cas9 Genom-Editing-Technologie, um das endogene Gen OsABCG15 zu knockouten, gefolgt von einem modifizierten Agrobacterium-vermitteltenTransformationsprotokoll, um eine stabile männlich-sterile Linie in Reis zu produzieren.

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