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Agrobacterium-Mediert genetisk transformasjon, transgen produksjon, og dens anvendelse for studiet av mannlig reproduktiv utvikling i ris
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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediert genetisk transformasjon, transgen produksjon, og dens anvendelse for studiet av mannlig reproduktiv utvikling i ris

DOI:
10.3791/61665-v

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07:43 min

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October 06, 2020

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, , , ,
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, 2College of Agronomy,Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine,University of Adelaide

Chapters

  • 00:04Introduction
  • 00:54Rice Genetic Transformation and Plant Tissue Culture
  • 04:00Observation of the Basic Phenotype of the Mutant
  • 05:14Results: Targeted Mutagenesis of the OsABCG15
  • 07:16Conclusion

Summary

Automatic Translation

Automatic Translation

Dette arbeidet beskriver bruken av CRISPR-Cas9 genomredigeringsteknologi for å slå ned endogene genet OsABCG15 etterfulgt av en modifisert Agrobacterium-medierttransformasjonsprotokoll for å produsere en stabil mannssteril linje i ris.

Tags

Agrobacterium-mediated TransformationTransgenic ProductionRiceMale Reproductive DevelopmentYoung InflorescencesCallus ProductionGene TransformationRegenerationVideo DemonstrationMeiosis StageNBD2 MediumEB PlateYEB MediumAAM AS MediumEmbryogenic Calli

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