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Journal / Biology / Agrobacterium-medierad genetisk omvandling,…
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JoVE Journal Biology
Agrobacterium-medierad genetisk omvandling, Transgen produktion, och dess tillämpning för studiet av manlig reproduktiv utveckling i Ris
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Agrobacterium-medierad genetisk omvandling, Transgen produktion, och dess tillämpning för studiet av manlig reproduktiv utveckling i Ris

Article DOI: 10.3791/61665-v • 07:43 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1, 1, 3, 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide
* These authors contributed equally

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Detta arbete beskriver användningen av CRISPR-Cas9 genomredigeringsteknik för att knockout endogena genen OsABCG15 följt av en modifierad Agrobacterium-medierad omvandling protokoll för att producera en stabil han-steril linje i ris.

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Denna månad i JoVE ris CRISPR-Cas9 Agrobacterium callus vävnadskultur genotyp fenotyp anther pollen
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