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Forberedelse af decellulariserede nyrestilladser hos rotter
JoVE Journal
Medicine
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JoVE Journal Medicine
Preparation of Decellularized Kidney Scaffolds in Rats
DOI:

06:09 min

March 18, 2021

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Chapters

  • 00:04Introduction
  • 01:00Preparation for Harvesting Rat Kidneys
  • 01:55Transcardial Perfusion
  • 03:22Kidney Harvesting and Decellularization
  • 04:37Results: Rat Kidneys Subjected to Renal Arterial Perfusion Decellularization
  • 05:35Conclusion

Summary

Automatic Translation

Denne protokol introducerer en metode til at udvikle et stillads ved hjælp af decellulariserede rotte nyrer. Protokollen indeholder decellulariserings- og recellulariseringsprocesser for at bekræfte biotilgængelighed. Decellularisering udføres ved hjælp af Triton X-100 og natrium dodecyl sulfat.

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