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1Department of Internal Medicine and Biomedical Research Institute,Pusan National University Hospital, 2Biomedical Research Institute,Pusan National University Hospital, 3Department of Plastic Surgery,Pusan National University Hospital, 4Department of Otorhinolaryngology – Head and Neck Surgery,Yangsan Pusan National University Hospital, 5Department of Otorhinolaryngology – Head and Neck Surgery,Pusan National University Hospital, 6Department of Pathology,Pusan National University Hospital
Chapters
- 00:04Introduction
- 01:00Preparation for Harvesting Rat Kidneys
- 01:55Transcardial Perfusion
- 03:22Kidney Harvesting and Decellularization
- 04:37Results: Rat Kidneys Subjected to Renal Arterial Perfusion Decellularization
- 05:35Conclusion
Denne protokol introducerer en metode til at udvikle et stillads ved hjælp af decellulariserede rotte nyrer. Protokollen indeholder decellulariserings- og recellulariseringsprocesser for at bekræfte biotilgængelighed. Decellularisering udføres ved hjælp af Triton X-100 og natrium dodecyl sulfat.
