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1Department of Internal Medicine and Biomedical Research Institute,Pusan National University Hospital, 2Biomedical Research Institute,Pusan National University Hospital, 3Department of Plastic Surgery,Pusan National University Hospital, 4Department of Otorhinolaryngology – Head and Neck Surgery,Yangsan Pusan National University Hospital, 5Department of Otorhinolaryngology – Head and Neck Surgery,Pusan National University Hospital, 6Department of Pathology,Pusan National University Hospital
Chapters
- 00:04Introduction
- 01:00Preparation for Harvesting Rat Kidneys
- 01:55Transcardial Perfusion
- 03:22Kidney Harvesting and Decellularization
- 04:37Results: Rat Kidneys Subjected to Renal Arterial Perfusion Decellularization
- 05:35Conclusion
Este protocolo introduz um método para desenvolver um andaime usando rins descelularizados. O protocolo inclui processos de descelularização e recelularização para confirmar a biodisponibilidade. A descelularização é realizada utilizando-se triton X-100 e sulfato de dodecyl de sódio.
