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Immunology and Infection
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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

DOI:
10.3791/61881-v

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05:37 min

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October 20, 2020

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, ,
1Molecular and Cellular Biology Department,University of Guelph

Chapters

  • 00:05Introduction
  • 01:14Macrophage Infection
  • 02:07Infection Verification
  • 03:04Sample Collection
  • 03:47Results: Representative Effects of C. neoformans Macrophage Infection
  • 05:04Conclusion

Summary

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Here, we present a protocol to profile the interplay between host and pathogen during infection by mass spectrometry-based proteomics. This protocol uses label-free quantification to measure changes in protein abundance of both host (e.g., macrophages) and pathogen (e.g., Cryptococcus neoformans) in a single experiment.

Tags

Label-freeQuantitative ProteomicsHost-pathogen InteractionsFungal InfectionMacrophage ResponseCryptococcusAnti-virulent StrategiesBioinformatics PipelineLDH AssayCytotoxicity

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