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JoVE Journal
Immunology and Infection

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Label-Free Kvantitative Proteomics Workflow for Discovery-Driven Host-Patogen Interaktioner
 
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Label-Free Kvantitative Proteomics Workflow for Discovery-Driven Host-Patogen Interaktioner

Article DOI: 10.3791/61881-v
October 20th, 2020

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Summary October 20th, 2020

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Her præsenterer vi en protokol til at profilere samspillet mellem vært og patogen under infektion med massespektrometri-baserede proteomics. Denne protokol bruger etiketfri kvantificering til at måle ændringer i proteintæthed for både vært (f.eks. makrofager) og patogen (f.eks. Cryptococcus neoformans)i et enkelt eksperiment.

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