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Journal
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Cancer Research
/
腫瘍増殖における癌関連線維芽細胞の役割を調べるためのマウスモデル
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth
Please note that all translations are automatically generated.
Click here for the English version.
腫瘍増殖における癌関連線維芽細胞の役割を調べるためのマウスモデル
DOI:
10.3791/61883-v
•
06:35 min
•
December 22, 2020
•
David Jelinek
2
,
Ellen Ran Zhang
2,3
,
Aaron Ambrus
2
,
Erin Haley
,
Emily Guinn
2
,
Austin Vo
2
,
Peter Le
2
,
Ayse Elif Kesaf
2
,
Jennifer Nguyen
2
,
Lily Guo
2
,
Destiny Frederick
2
,
Zhengyang Sun
2
,
Natalie Guo
,
Parker Sevier
2
,
Eric Bilotta
2
,
Kaiser Atai
2,4
,
Laurent Voisin
2
,
Hilary A. Coller
2,4
1
Department of Molecular, Cell and Developmental Biology
,
University of California, Los Angeles
,
2
Department of Biological Chemistry, David Geffen School of Medicine
,
University of California, Los Angeles
,
3
Department of Molecular Biology
,
Princeton University
,
4
Molecular Biology Institute
,
University of California, Los Angeles
Chapters
00:05
Introduction
00:43
Plating the Cancer Cells
01:56
Preparation of Cancer Cells and Fibroblasts for Injection into Mice
04:13
Inject Cancer Cells and Fibroblasts into Mice
05:21
Results: Analysis of Cancer-Associated Fibroblasts in Tumor Growth in Mice
06:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
癌細胞と線維芽細胞を同時注入し、腫瘍の成長を経時的に監視するためのプロトコルが提供されます。このプロトコルは、腫瘍増殖の調節因子としての線維芽細胞の役割の分子基盤を理解するために使用できます。
Tags
Cancer-associated Fibroblasts
Tumor Growth
Melanoma
Mouse Model
Cell Culture
Trypsin-EDTA
Hemocytometer
Cell Counting
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