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Immunology and Infection
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Mikrofluidiske cokulturmodeller til dissekering af immunresponset i in vitro tumormikromiljøer
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Immunology and Infection
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JoVE Journal Immunology and Infection
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Mikrofluidiske cokulturmodeller til dissekering af immunresponset i in vitro tumormikromiljøer

DOI:
10.3791/61895-v

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07:46 min

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April 30, 2021

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, , , , , , , ,
1CNR Institute for Photonics and Nanotechnology, 2Dept. of Oncology and Molecular Medicine,Istituto Superiore di Sanità, 3Istituto di Patologia Generale,Università Cattolica del Sacro Cuore, 4Tumor Immunology and Immunotherapy Unit,IRCCS Regina Elena National Cancer Institute

Chapters

  • 00:04Introduction
  • 01:022D Chip Cell Plating
  • 02:10Unlabeled Cancer and Immune Cell Time-Lapse Acquisition
  • 02:433D Immuno-Competent Cancer On-Chip Model
  • 05:15Results I: Migratory and Interaction Patterns of the 2D Tumor-Immune Cells Co-Culture by Time-Lapse Data Analysis
  • 06:19Results II: Fluorescent Analysis of Infiltrated Immune Cells in a 3D Tumor Microenvironment
  • 06:56Conclusion

Summary

Automatic Translation

Automatic Translation

I en tid med immunterapi og enkeltcellegenomisk profilering kræver kræftbiologi nye in vitro- og beregningsværktøjer til undersøgelse af tumor-immungrænsefladen i en ordentlig rumlig tidsmæssig sammenhæng. Vi beskriver protokoller til udnyttelse af tumorimmune mikrofluidiske co-kulturer i 2D- og 3D-indstillinger, kompatible med dynamisk, multiparametrisk overvågning af cellulære funktioner.

Tags

MicrofluidicCo-cultureTumor MicroenvironmentImmune ResponseCell Interactions2D3DReal-time ImagingMicroscopyTumor CellsImmune CellsAnti-cancer TreatmentsMatrigelFluorescent Dyes

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