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维持静止人类造血干细胞的文化方法
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
维持静止人类造血干细胞的文化方法
DOI:
10.3791/61938-v
•
07:14 min
•
May 17, 2021
•
Hiroshi Kobayashi
,
Keiyo Takubo
1
Department of Stem Cell Biology, Research Institute
,
National Center for Global Health and Medicine
Chapters
00:04
Introduction
01:16
Lipid Preparation
02:13
Media Preparation
03:43
Cell Culture
04:47
Results: Cell Numbers After 7 Days of Culturing
06:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议通过使用丰富的脂肪酸、胆固醇、低浓度的细胞因子和缺氧来模拟骨髓微环境,使静默的人类造血干细胞在体外得以维持。
Tags
Hematopoietic Stem Cells
Quiescence
Cell Cycle
In Vitro Culture
Anti-cancer Drugs
Lipid Solution
Culture Media
Stem Cell Factor
Thrombopoietin
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