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Bioengineering

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JoVE Journal Bioengineering
Un metodo di coltura per mantenere le cellule staminali ematopoietiche umane quiescenti
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Un metodo di coltura per mantenere le cellule staminali ematopoietiche umane quiescenti

Article DOI: 10.3791/61938-v • 07:14 min • May 17th, 2021
May 17th, 2021 •
1, 1
1Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine

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Questo protocollo consente il mantenimento in vitro di cellule staminali ematopoietiche umane quiescenti imitando il microambiente del midollo osseo utilizzando abbondanti acidi grassi, colesterolo, concentrazioni più basse di citochine e ipossia.

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Bioingegneria Numero 171 Cellule staminali ematopoietiche quiescente acido grasso colesterolo ipossia microambiente del midollo osseo
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