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Journal / Chemistry / Kovavt märkning med Diethylpyrocarbonate för…
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Kovavt märkning med Diethylpyrocarbonate för studier av protein högre order struktur av masspektrometri
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Kovavt märkning med Diethylpyrocarbonate för studier av protein högre order struktur av masspektrometri

Article DOI: 10.3791/61983-v • 10:36 min • June 15th, 2021
June 15th, 2021 •
1, 1, *1,2, *3
1Department of Chemistry, University of Massachusetts Amherst, 2Molecular and Cellular Biology Program, University of Massachusetts Amherst, 3Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University
* These authors contributed equally

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De experimentella förfarandena för att utföra diethylpyrocarbonate-baserade kovalement märkning med massa spektroskopisk detektion beskrivs. Diethylpyrocarbonat blandas helt enkelt med protein- eller proteinkomplexet av intresse, vilket leder till modifiering av lösningsmedelskomliga aminosyrarester. De modifierade resterna kan identifieras efter proteolytisk matsmältning och vätskekromatografi/masspektrometrianalys.

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Kemi Utgåva 172 Masspektrometri kovamerad märkning proteinstruktur med högre ordning protein-ligand komplex proteinkomplex diethylpyrocarbonat lösningsmedelsanpassad yta
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