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Bioengineering
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逐步在泰瑟尔脚手架上播种,研究发芽血管
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
Please note that all translations are automatically generated.
Click here for the English version.
逐步在泰瑟尔脚手架上播种,研究发芽血管
DOI:
10.3791/61995-v
•
07:49 min
•
January 14, 2021
•
Ariel A. Szklanny
,
Dylan B. Neale
,
Joerg Lahann
,
Shulamit Levenberg
1
Faculty of Biomedical Engineering
,
Technion
,
2
Department of Chemical Engineering and Biointerfaces Institute
,
University of Michigan, Ann Arbor
Chapters
00:04
Introduction
00:50
Scaffold Fibronectin Coating
01:47
Endothelial Cells Seeding
02:57
Support Cell Seeding and Co-Culture
05:31
Results: Analysis of Organized Vascular Networks on Tessellated Scaffold Geometries
07:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
工程组织严重依赖适当的血管网络来提供重要的营养和气体,并清除代谢废物。在这项工作中,内皮细胞和支持细胞的分步播种协议在高通量平台中创建高度有序的血管网络,用于研究在受控的 3D 环境中开发血管行为。
Tags
Keywords: Cell Seeding
Tessellated Scaffolds
Blood Vessels
Vascular Networks
Endothelial Cells
Fibronectin
Cell Culture
Cell Suspension
Cell Incubation
Cell Imaging
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