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Biology
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小尺寸远程模型日本美达卡(
奥里齐亚斯拉蒂普斯
)的果纳切除术和血液采样程序
JoVE Journal
Biology
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JoVE Journal
Biology
Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (
Oryzias latipes
)
Please note that all translations are automatically generated.
Click here for the English version.
小尺寸远程模型日本美达卡(
奥里齐亚斯拉蒂普斯
)的果纳切除术和血液采样程序
DOI:
10.3791/62006-v
•
05:31 min
•
December 11, 2020
•
Muhammad Rahmad Royan
,
Shinji Kanda
,
Daichi Kayo
,
Weiyi Song
,
Wei Ge
,
Finn-Arne Weltzien
,
Romain Fontaine
1
Physiology Unit, Faculty of Veterinary Medicine
,
Norwegian University of Life Sciences
,
2
Laboratory of Physiology, Atmosphere and Ocean Research Institute
,
The University of Tokyo
,
3
Department of Biological Sciences, Graduate School of Science
,
The University of Tokyo
,
4
Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences
,
University of Macau
Chapters
00:04
Introduction
00:50
Gonadectomy
02:22
Blood Sampling
03:16
Results: E2 and 11-KT Levels in Females and Males of Sham-Operated and Gonadectomized Fish
04:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文描述了一个快速协议去纳切除术和采样血液从小远程鱼,使用日本美达卡
(Oryzias拉蒂普斯
)作为模型,以调查性类固醇在动物生理中的作用。
Tags
Gonadectomy
Blood Sampling
Japanese Medaka
Teleost
Ovariectomy
Orchidectomy
Anesthesia
Incision
Suturing
Blood Draw
Caudal Peduncle Vein
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