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使用微生理系统模拟人体乳房组织中的乳腺癌
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Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
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使用微生理系统模拟人体乳房组织中的乳腺癌
DOI:
10.3791/62009-v
•
10:51 min
•
April 23, 2021
•
Loren M. Brown
,
Katherine L. Hebert
,
Rakesh R. Gurrala
,
C. Ethan Byrne
,
Matthew Burow
,
Elizabeth C. Martin
,
Frank H. Lau
1
Department of Surgery
,
Louisiana State University Health Sciences Center
,
2
Department of Bioinnovation
,
Tulane University
,
3
Tulane University School of Medicine
,
4
Department of Biological and Agricultural Engineering
,
Louisiana State University
,
5
Department of Medicine
,
Tulane University School of Medicine
Chapters
00:04
Introduction
01:10
Preparation of Gelatin Plungers and Application to the Upper Cell Sheets
02:21
Processing of Human Breast Tissue
05:21
Digestion of BC-MPS for Analysis
08:09
Results: Analysis of BC-MPS Culture
10:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了使用原始人体乳房组织与现成材料研究乳腺癌的体外微生理系统的构建。
Tags
Breast Cancer
Microphysiological System
Ex Vivo
Adipocytes
Immune Cells
Vascular Structures
Ductal Structures
Extracellular Matrix
Personalized Medicine
Pharmaceutical Development
Tumor Initiation
Fibrosis
Extracellular Matrix Remodeling
Gelatin
PNIPAAm
ASCs
PBS
Razor Blade
Cancer Cell Lines
BC-MPS Media
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