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Journal
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Cancer Research
/
微小生理学的システムを用いたヒト乳房組織における乳癌のモデル化
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JoVE Journal
Cancer Research
Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
Please note that all translations are automatically generated.
Click here for the English version.
微小生理学的システムを用いたヒト乳房組織における乳癌のモデル化
DOI:
10.3791/62009-v
•
10:51 min
•
April 23, 2021
•
Loren M. Brown
,
Katherine L. Hebert
,
Rakesh R. Gurrala
,
C. Ethan Byrne
,
Matthew Burow
,
Elizabeth C. Martin
,
Frank H. Lau
1
Department of Surgery
,
Louisiana State University Health Sciences Center
,
2
Department of Bioinnovation
,
Tulane University
,
3
Tulane University School of Medicine
,
4
Department of Biological and Agricultural Engineering
,
Louisiana State University
,
5
Department of Medicine
,
Tulane University School of Medicine
Chapters
00:04
Introduction
01:10
Preparation of Gelatin Plungers and Application to the Upper Cell Sheets
02:21
Processing of Human Breast Tissue
05:21
Digestion of BC-MPS for Analysis
08:09
Results: Analysis of BC-MPS Culture
10:00
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
このプロトコルは、貯蔵材料を使った原発性ヒト乳房組織を用いて乳癌を研究するためのインビトロ微小生理学的システムの構築を記述する。
Tags
Breast Cancer
Microphysiological System
Ex Vivo
Adipocytes
Immune Cells
Vascular Structures
Ductal Structures
Extracellular Matrix
Personalized Medicine
Pharmaceutical Development
Tumor Initiation
Fibrosis
Extracellular Matrix Remodeling
Gelatin
PNIPAAm
ASCs
PBS
Razor Blade
Cancer Cell Lines
BC-MPS Media
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