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使用从纤维细胞直接转换为神经元祖先细胞和分化为星形细胞的神经系统疾病体外建模
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
Please note that all translations are automatically generated.
Click here for the English version.
使用从纤维细胞直接转换为神经元祖先细胞和分化为星形细胞的神经系统疾病体外建模
DOI:
10.3791/62016-v
•
11:42 min
•
June 10, 2021
•
Cassandra N. Dennys
,
Julieth A. Sierra-Delgado
,
Shrestha Sinha Ray
,
Annalisa M. Hartlaub
,
Florence S. Roussel
,
Yacidzohara Rodriguez
,
Kathrin Meyer
2
1
Center for Gene Therapy
,
The Research Institute at Nationwide Children’s Hospital
,
2
College of Medicine, The Ohio State University
Chapters
00:00
Introduction
01:01
Direct Conversion of Human Fibroblasts to Neuronal Progenitor Cells
04:21
Conversion and iNPC Splitting
06:42
Generating Induced Astrocytes From NPCs
07:43
Astrocyte Differentiation From Frozen iNPC Stocks
08:51
Results: Growth and Characterization of Cells Throughout Conversion Process
10:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个将人类皮肤衍生的成纤维细胞重新编程为诱导神经元祖先细胞(iNPC)的协议,以及它们随后分化成诱导的星形细胞(iAs)的协议。这种方法可快速、可重复地大量生成 iNC 和 iAs。
Tags
In Vitro Modeling
Neurological Diseases
Fibroblasts
Neuronal Progenitor Cells
Astrocytes
Direct Conversion
Skin Biopsy
Retroviral Vectors
Oct3/4
Klf4
Sox2
C-Myc
Transduction
Neurodegenerative Diseases
Cell Culture
Co-cultures
Disease Mechanisms
Medication Testing
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