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Journal
/
Biochemistry
/
贝克酵母糖精
中
线粒体基因组表达产品的标记和可视化
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
贝克酵母糖精
中
线粒体基因组表达产品的标记和可视化
DOI:
10.3791/62020-v
•
08:33 min
•
April 11, 2021
•
Ivan V. Chicherin
2
,
Sergei A. Levitskii
,
Maria V. Baleva
,
Igor A. Krasheninnikov
,
Maxim V. Patrushev
,
Piotr A. Kamenski
1
Department of Biology
,
M.V. Lomonosov Moscow State University
,
2
Institute of Functional Genomics
,
M.V. Lomonosov Moscow State University
,
3
National Research Centre “Kurchatov Institute”
Chapters
00:00
Introduction
00:39
Preparation of Yeast Culture
02:10
Radioactive Isotope Incorporation
03:34
Yeast Cell Lysis and Extraction of Proteins
04:20
Precipitation of Proteins
05:15
Electrophoretic Separation
05:43
Autoradiography
05:59
Representative Results
07:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
贝克的酵母线粒体基因组编码了八种多肽。当前协议的目标是将所有这些都标记为单独的频段,然后将其可视化为单独的频段。
Tags
Mitochondrial Genome
Mitochondrial Protein Biosynthesis
Radioactive Labeling
Gel Electrophoresis
Saccharomyces Cerevisiae
Yeast
Translation
Cycloheximide
S35 Methionine
Borymycin
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