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Developmental Biology
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単一の卵母細胞レポーターアッセイを用いたインビトロ成熟時の母体母体翻訳プログラムの定義
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Developmental Biology
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JoVE Journal
Developmental Biology
Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Please note that all translations are automatically generated.
Click here for the English version.
単一の卵母細胞レポーターアッセイを用いたインビトロ成熟時の母体母体翻訳プログラムの定義
DOI:
10.3791/62041-v
•
08:00 min
•
June 16, 2021
•
Natasja G. J. Costermans
2
,
Enrico M. Daldello
2,3
,
Ria J. Marathe
2
,
Marco Conti
2
1
Center for Reproductive Sciences
,
University of California at San Francisco
,
2
Department of Obstetrics Gynecology and Reproductive Sciences
,
University of California at San Francisco
,
3
Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS
,
Sorbonne Université
Chapters
00:04
Introduction
00:38
Aspiration of Denuded Oocyte and its Microinjection
03:18
Time-lapse Microscopy and Analysis of Ypet-3 UTR Translation
06:00
Results: Statistical Analysis of the Expression for Reporter-YFP and mCherry in Prophase I and Maturing Oocytes
07:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルは、
インビトロ
成熟時の単一卵母細胞におけるmRNA翻訳の調節を研究するためのレポーターアッセイを記述する。
Tags
Maternal MRNA Translation
In Vitro Oocyte Maturation
Single Oocyte Reporter Assay
Time-lapse Microscopy
Reporter Accumulation
Translational Activation
Translational Repression
Antral Follicle
Cumulus-oocyte Complex (COC)
Denuding
Cilostamide
Microinjection
MCherry
Ypet
Time-lapse Imaging
Article
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