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JoVE Journal Biology
Seriel blok-ansigt scanning elektron mikroskopi (SBF-SEM) af biologiske vævsprøver
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Seriel blok-ansigt scanning elektron mikroskopi (SBF-SEM) af biologiske vævsprøver

Article DOI: 10.3791/62045-v • 09:21 min • March 26th, 2021
March 26th, 2021 •
1, 1, 1, 2, 2, 1, 3,4, 1,4
1College of Optometry, University of Houston, 2Department of Biology, DePauw University, 3Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey Veterans Affairs Medical Center, 4Children’s Nutrition Center, Baylor College of Medicine

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Denne protokol skitserer en rutinemæssig metode til brug af seriel blok-ansigt scanning elektronmikroskopi (SBF-SEM), en kraftfuld 3D-billedbehandling teknik. Vellykket anvendelse af SBF-SEM afhænger af korrekt fiksering og væv farvning teknikker, samt omhyggelig overvejelse af billedbehandling indstillinger. Denne protokol indeholder praktiske overvejelser for hele denne proces.

Tags

Biologi Problem 169 Seriel blok-ansigt Scanning Elektron Mikroskopi SBF-SEM 3D-EM 3D-Rekonstruktion Vævsforberedelse Prøve Forberedelse Seriel sektioner Ultrastruktur Høj opløsning Volumetrisk Imaging Biologisk Stor volumen
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