Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Seriell block-face scanning elektronmikroskopi…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Seriell block-face scanning elektronmikroskopi (SBF-SEM) av biologiska vävnadsprover
Developing a Tool for Using Inverted Confocal Microscopes for In Vivo Intravital Imaging (Video) | JoVE
Journal (Biology)
Developing a Tool for Using Inverted Confocal Microscopes for In Vivo Intravital Imaging (Video) | JoVE
Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)
Journal (Biology)
Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)
 
Click here for the English version

Seriell block-face scanning elektronmikroskopi (SBF-SEM) av biologiska vävnadsprover

Article DOI: 10.3791/62045-v • 09:21 min • March 26th, 2021
March 26th, 2021 •
1, 1, 1, 2, 2, 1, 3,4, 1,4
1College of Optometry, University of Houston, 2Department of Biology, DePauw University, 3Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey Veterans Affairs Medical Center, 4Children’s Nutrition Center, Baylor College of Medicine

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Detta protokoll beskriver en rutinmetod för att använda seriell block-face scanning elektronmikroskopi (SBF-SEM), en kraftfull 3D-bildteknik. Framgångsrik tillämpning av SBF-SEM beror på korrekt fixering och vävnad färgning tekniker, samt noggrant övervägande av bildinställningar. Detta protokoll innehåller praktiska överväganden för hela denna process.

Tags

Biologi utgåva 169 Seriell block-face scanning elektronmikroskopi SBF-SEM 3D-EM 3D-rekonstruktion vävnadsberedning provberedning seriella sektioner ultrastruktur högupplöst volymetrisk avbildning biologisk stor volym
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter