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Journal / Immunology and Infection / Immuno RNA-Floresan In Situ Hibridizasyonu…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Immuno RNA-Floresan In Situ Hibridizasyonu Kullanılarak SARS-CoV-2'nin Görselleştirilmesi
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Immuno RNA-Floresan In Situ Hibridizasyonu Kullanılarak SARS-CoV-2'nin Görselleştirilmesi

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Burada, şiddetli akut solunum sendromu coronavirus 2 (SARS-CoV-2) RNA'yı görselleştirmek için RNA floresan in situ hibridizasyonunu (RNA-FISH) immünofluoresans ile birleştiren basit bir yöntemi açıklıyoruz. Bu protokol, SARS-CoV-2 RNA konakçı etkileşimlerinin moleküler özelliklerinin tek hücre düzeyinde anlaşılmasını artırabilir.

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İmmünoloji ve Enfeksiyon Sayı 166 SARS-CoV-2 RNA-FISH Hibridizasyon zinciri reaksiyonu İmmünofluoresans Human Airway Epitel (HAE) Permeabilizasyon Konfokal mikroskopi
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