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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisatie van SARS-CoV-2 met behulp van Immuno RNA-Fluorescentie In Situ Hybridisatie
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Visualisatie van SARS-CoV-2 met behulp van Immuno RNA-Fluorescentie In Situ Hybridisatie

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Hier beschrijven we een eenvoudige methode die RNA-fluorescentie in situ hybridisatie (RNA-FISH) combineert met immunofluorescentie om ernstig acuut respiratoir syndroom coronavirus 2 (SARS-CoV-2) RNA te visualiseren. Dit protocol kan het begrip van de moleculaire kenmerken van SARS-CoV-2 RNA-host interacties op eencellig niveau vergroten.

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Immunologie en infectie SARS-CoV-2 RNA-FISH Hybridisatiekettingreactie Immunofluorescentie Human Airway Epithelium (HAE) Permeabilisatie Confocale microscopie
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