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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisering av SARS-CoV-2 ved hjelp av Immuno RNA-Fluorescence In Situ Hybridization
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Visualisering av SARS-CoV-2 ved hjelp av Immuno RNA-Fluorescence In Situ Hybridization

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Her beskriver vi en enkel metode som kombinerer RNA fluorescens in situ hybridisering (RNA-FISH) med immunfluorescens for å visualisere alvorlig akutt respiratorisk syndrom coronavirus 2 (SARS-CoV-2) RNA. Denne protokollen kan øke forståelsen av de molekylære egenskapene til SARS-CoV-2 RNA-vert interaksjoner på et enkeltcellet nivå.

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Immunologi og infeksjon utgave 166 SARS-CoV-2 RNA-FISH Hybridiseringskjedereaksjon Immunfluorescence Human Airway Epithelium (HAE) Permeabilization Konfiskert mikroskopi
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