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Journal
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Medicine
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使用荧光标记肉瘤蛋白缩短多能干细胞衍生心肌细胞的肉瘤。
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Medicine
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JoVE Journal
Medicine
Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
Please note that all translations are automatically generated.
Click here for the English version.
使用荧光标记肉瘤蛋白缩短多能干细胞衍生心肌细胞的肉瘤。
DOI:
10.3791/62129-v
•
08:37 min
•
March 03, 2021
•
Razan E. Ahmed*
1
,
Nawin Chanthra*
1
,
Tatsuya Anzai
2
,
Keiichiro Koiwai
4
,
Tomoki Murakami
,
Hiroaki Suzuki
,
Yutaka Hanazono
,
Hideki Uosaki
1
Division of Regenerative Medicine, Center for Molecular Medicine
,
Jichi Medical University
,
2
Department of Pediatrics
,
Jichi Medical University
,
3
Institute of Global Innovation Research
,
Tokyo University of Agriculture and Technology
,
4
Department of Precision Mechanics, Faculty of Science and Engineering
,
Chuo University
Chapters
00:04
Introduction
01:04
Human Pluripotent Stem Cell Differentiation
03:15
Patterned Pluripotent Stem Cell-Derived Cardiomyocyte (PSC-CM) Culture
05:07
Time-Lapse Sarcomere Imaging
05:58
Time-Lapse Imaging Analysis
06:36
Results: Representative Sarcomere Shortening Evaluation
07:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方法可用于使用多能干细胞衍生心肌细胞与荧光标记肉瘤蛋白来检查肉瘤缩短。
Tags
Sarcomere Shortening
Pluripotent Stem Cell-derived Cardiomyocytes
Fluorescent-tagged Sarcomere Proteins
AAV-based Transduction
Induced Pluripotent Stem Cells
Cardiomyopathy Therapy
Skeletal Muscle Dysfunction
Myopathy
Laminin-511 E8
ROCK Inhibitor
GSK-3 Inhibitor
Porcupine Inhibitor
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