JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
蛍光タグ付きサルコメアタンパク質を用いた多能性幹細胞由来心筋細胞のサルコメア短縮
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
Please note that all translations are automatically generated.
Click here for the English version.
蛍光タグ付きサルコメアタンパク質を用いた多能性幹細胞由来心筋細胞のサルコメア短縮
DOI:
10.3791/62129-v
•
08:37 min
•
March 03, 2021
•
Razan E. Ahmed*
1
,
Nawin Chanthra*
1
,
Tatsuya Anzai
2
,
Keiichiro Koiwai
4
,
Tomoki Murakami
,
Hiroaki Suzuki
,
Yutaka Hanazono
,
Hideki Uosaki
1
Division of Regenerative Medicine, Center for Molecular Medicine
,
Jichi Medical University
,
2
Department of Pediatrics
,
Jichi Medical University
,
3
Institute of Global Innovation Research
,
Tokyo University of Agriculture and Technology
,
4
Department of Precision Mechanics, Faculty of Science and Engineering
,
Chuo University
Chapters
00:04
Introduction
01:04
Human Pluripotent Stem Cell Differentiation
03:15
Patterned Pluripotent Stem Cell-Derived Cardiomyocyte (PSC-CM) Culture
05:07
Time-Lapse Sarcomere Imaging
05:58
Time-Lapse Imaging Analysis
06:36
Results: Representative Sarcomere Shortening Evaluation
07:56
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
この方法は、蛍光タグ付きサルコメアタンパク質を有する多能性幹細胞由来心筋細胞を用いてサルコメア短縮を調べるために使用することができる。
Tags
Sarcomere Shortening
Pluripotent Stem Cell-derived Cardiomyocytes
Fluorescent-tagged Sarcomere Proteins
AAV-based Transduction
Induced Pluripotent Stem Cells
Cardiomyopathy Therapy
Skeletal Muscle Dysfunction
Myopathy
Laminin-511 E8
ROCK Inhibitor
GSK-3 Inhibitor
Porcupine Inhibitor
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
中リコンビナーゼ媒介カセット交換による組換えヒト多能性幹細胞の迅速かつ効率的な生成<em> AAVS1</em>軌跡
ラット心筋細胞の分離と培養
小動物モデルにおける外部ビーム放射線を用いた多能性幹細胞由来の奇形の対象となる、選択的な治療
細胞外フラックス アナライザーを使用してプライマリの培養マウス新生児心筋酸素消費量の分析
皮膚心筋細胞を用いた心機能のインビトロ評価
hiPSC由来心筋細胞を用いた虚血性心疾患のモデルと心筋細胞収縮の映像的比較
ヒト多能性幹細胞からの網膜オルガノイドの誘導
ヒト多能性幹細胞からの3Dレチナル組織の導出のためのレチナルオガノイド誘導システム
Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice
ヒト人工多能性幹細胞由来心筋細胞における微小電極アレイおよびパッチクランプ記録の技術的応用
Read Article