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Biology

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En 3D-kartografisk beskrivelse af cellen ved Hjælp af Cryo Soft X-ray Tomography
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En 3D-kartografisk beskrivelse af cellen ved Hjælp af Cryo Soft X-ray Tomography

Article DOI: 10.3791/62190-v • 08:47 min • March 15th, 2021
March 15th, 2021 •
1, 1, 1, 1, 1, 2, 2, 2, 1, 1
1MISTRAL beamline, Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

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Her præsenteres en protokol, der beskriver de prøveforberedelses- og dataindsamlingstrin, der kræves i kryo blød røntgentomografi (SXT) for at afbilde ultrastrukturen af hele kryokonserverede celler i en opløsning på 25 nm halv tonehøjde.

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Biologi udgave 169 røntgenbilleddannelse blød røntgentomografi kryogene temperaturer helcelle spektromikroskopi
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