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iPSC-巨噬细胞死亡神经母细胞的体外定量成像检测
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
Please note that all translations are automatically generated.
Click here for the English version.
iPSC-巨噬细胞死亡神经母细胞的体外定量成像检测
DOI:
10.3791/62217-v
•
09:48 min
•
February 14, 2021
•
Hazel Hall-Roberts
2,3
,
Elena Di Daniel
,
William S. James
,
John B. Davis
,
Sally A. Cowley
1
James Martin Stem Cell Facility, Sir William Dunn School of Pathology
,
University of Oxford
,
2
Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building
,
University of Oxford
,
3
UK Dementia Research Institute
,
Cardiff University
Chapters
00:04
Introduction
00:41
Preparation of Dead SH-SY5Ys
02:03
Labeling of Dead SH-SY5Ys with pH-Sensitive Red Fluorescent Dye
03:18
Staining of iPSC-Macrophages
04:50
Fixed-Cell High-Content Imaging
07:28
Results: Imaging, Optimization and Validation of Phagocytosis Assay
09:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
神经退行性疾病与受管制的微胶质功能障碍有关。本文概述了iPSC巨噬细胞对神经母细胞的体外测定。定量显微镜读数用于活细胞延时成像和固定细胞高含量成像。
Tags
In Vitro
Quantitative Imaging Assay
Phagocytosis
Dead Neuroblastoma Cells
IPSC-macrophages
Neurodegenerative Disease
SH-SY5Y Cells
Cell Dissociation
Fixation
PH-sensitive Dye
Fluorescent Dye
Macrophage Media
Hoechst 33342
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