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在体内 小鼠劣质橄榄中的钙成像
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal Neuroscience
In vivo Calcium Imaging in Mouse Inferior Olive
DOI:

08:58 min

June 10, 2021

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Chapters

  • 00:00Introduction
  • 00:57Protocol
  • 01:45Administration of Anesthesia and Preparation of the Mouse for the Surgery
  • 02:42Tracheotomy and Intubation
  • 05:02Exposing the Brainstem
  • 06:42Calcium Imagin
  • 07:55Representative Results
  • 08:20Conclusion

Summary

Automatic Translation

我们提出一个协议,从心室侧暴露成年小鼠的脑系统。通过使用带微型显微镜的梯度折射指数透镜,钙成像可用于检查体内低劣橄榄神经索玛塔的活性。

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