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Journal / Biology / 通过病毒样颗粒（"纳米刀片"）在永生化和原代细胞中递送Cas9 /…
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Biology

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JoVE Journal Biology
通过病毒样颗粒（"纳米刀片"）在永生化和原代细胞中递送Cas9 / sgRNA核糖核蛋白复合物
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Journal (Biology)
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
 
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通过病毒样颗粒（"纳米刀片"）在永生化和原代细胞中递送Cas9 / sgRNA核糖核蛋白复合物

Article DOI: 10.3791/62245-v • 09:23 min • March 31st, 2021
March 31st, 2021 •
2, 1, 1, 1
1LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, 2CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France

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Summary
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我们开发了一种简单且廉价的方案，可在病毒样颗粒中加载Cas9 /单向导RNA（sgRNA）核糖核蛋白复合物。这些被称为"纳米刀片"的颗粒允许在不朽和原代细胞以及体内有效地递送Cas9 / sgRNA复合物。

Tags

生物学，第169期，CRISPR，Cas9，核糖核蛋白，RNP，鼠白血病病毒，病毒样颗粒，VLP，病毒载体，蛋白质递送
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