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Journal / Biology / Somministrazione del complesso ribonucleoproteico …
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JoVE Journal Biology
Somministrazione del complesso ribonucleoproteico Cas9/sgRNA in cellule immortalizzate e primarie tramite particelle simili a virus ("Nanoblades")
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Somministrazione del complesso ribonucleoproteico Cas9/sgRNA in cellule immortalizzate e primarie tramite particelle simili a virus ("Nanoblades")

Article DOI: 10.3791/62245-v • 09:23 min • March 31st, 2021
March 31st, 2021 •
2, 1, 1, 1
1LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, 2CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France

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Abbiamo sviluppato un protocollo semplice ed economico per caricare complessi di ribonucleoproteine Cas9/single-guide RNA (sgRNA) all'interno di particelle simili a virus. Queste particelle, chiamate "Nanoblades", consentono una consegna efficiente del complesso Cas9/sgRNA in cellule immortalizzate e primarie e in vivo.

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Biologia Numero 169 CRISPR Cas9 ribonucleoproteina RNP Virus della leucemia murina Particelle simili a virus VLP vettore virale consegna di proteine
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