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使用新型机器人系统 Spotiton 进行时间解决研究的低温电子微观网格准备
JoVE Journal
Biology
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JoVE Journal
Biology
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
Please note that all translations are automatically generated.
Click here for the English version.
使用新型机器人系统 Spotiton 进行时间解决研究的低温电子微观网格准备
DOI:
10.3791/62271-v
•
08:59 min
•
February 25, 2021
•
William C. Budell
,
Luis Allegri
,
Venkat Dandey
,
Clinton S. Potter
,
Bridget Carragher
1
The National Resource for Automated Molecular Microscopy
,
Simons Electron Microscopy Center, New York Structural Biology Center
,
2
piTree Software
Chapters
00:04
Introduction
00:49
Set Up the Spotiton Machine and Software
03:40
Prepare Two-sample, Mixed Grids
07:09
Results: System Camera Images and the Pattern of Ice Deposition on the Spotiton-prepared Grid
08:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里提出的协议描述了使用Spotiton,一种新型的机器人系统,将两个感兴趣的样品送到一个自吸的纳米线网格上,在液体低温素的化之前混合至少90ms。
Tags
Cryo-electron Microscopy
Spotiton
Robotic System
Time-resolved Studies
Protein Conformation
Membrane Channel Activation
DNA/RNA Synthesis
Drug/antibody Interaction
Grid Preparation
Nitrogen Supply
Water Reservoir
Robot Initialization
Tip Priming
Droplet Inspection
Grid Loading
Plunge Path Camera
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