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Journal
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Biology
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低温结构照明微观数据收集来自低温保存细胞
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
Please note that all translations are automatically generated.
Click here for the English version.
低温结构照明微观数据收集来自低温保存细胞
DOI:
10.3791/62274-v
•
11:55 min
•
May 28, 2021
•
Nina Vyas*
1
,
Nina Perry*
1
,
Chidinma A. Okolo
,
Ilias Kounatidis
,
Thomas M. Fish
,
Kamal L. Nahas
2
,
Archana Jadhav
,
Mohamed A. Koronfel
,
Johannes Groen
,
Eva Pereiro
,
Ian M. Dobbie
,
Maria Harkiolaki
1
Harwell Science and Innovation Campus
,
Beamline B24, Diamond Light Source
,
2
Division of Virology, Department of Pathology
,
University of Cambridge
,
3
ALBA Synchrotron
,
Beamline 09 – MISTRAL
,
4
Micron Advanced Imaging Consortium, Department of Biochemistry
,
University of Oxford
Chapters
00:04
Introduction
01:09
Preparation of the Cryo-Stage
02:08
Transfer of the Sample Storage Box into the Cryo-Stage
03:34
Stage Docking and Focusing
05:03
Brightfield Mosaic Acquisition and Identification of Areas of Interest
06:48
Data Collection
08:59
After Imaging
09:38
Results: Cryo-structured Illumination Microscopic Imaging
11:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议演示了如何使用低温结构照明显微镜对生物低温保存样品进行成像。我们通过成像 U2OS 细胞的细胞骨骼来演示该方法。
Tags
Cryo-structured Illumination Microscopy
Super Resolution Cryo Imaging
Cellular Ultrastructure
Vaccine Production
Antibody Engineering
Nano Particle Characterization
Cryo-stage
Liquid Nitrogen
Sample Transfer Cassette
TEM Grid
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