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Journal / Biology / 極低温保存細胞からの微視的データ収集…
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Biology

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JoVE Journal Biology
極低温保存細胞からの微視的データ収集
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極低温保存細胞からの微視的データ収集

Article DOI: 10.3791/62274-v • 11:55 min • May 28th, 2021
May 28th, 2021 •
*1, *1, 1, 1, 1, 1,2, 1, 1, 3, 3, 4, 1
1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford
* These authors contributed equally

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このプロトコルは、クライオ構造の照明顕微鏡を用いて、生物学的に凍結保存されたサンプルを画像化する方法を示す。U2OS細胞の細胞骨格をイメージングして方法論を実証する。

Tags

生物学,課題 171 低温イメージング 蛍光顕微鏡 3D画像法 超分解能顕微鏡 クライオSIM 構造化照明顕微鏡 ココラ性画像
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