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注射重组狐尾花叶病毒和甘蔗花叶病毒传染性克隆直接农种玉米幼苗
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
Please note that all translations are automatically generated.
Click here for the English version.
注射重组狐尾花叶病毒和甘蔗花叶病毒传染性克隆直接农种玉米幼苗
DOI:
10.3791/62277-v
•
05:56 min
•
February 27, 2021
•
Bliss M. Beernink*
1
,
Katerina L. Holan*
1
,
Ryan R. Lappe
,
Steven A. Whitham
1
Department of Plant Pathology and Microbiology
,
Iowa State University
Chapters
00:04
Introduction
00:39
Preparation of Seedling and
Agrobacterium
02:06
Injection of
Agrobacterium
and Infection Detection
03:36
Results: Detection of Infection Lesion and Gene Suppression Analysis
05:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了一种基于
农杆菌
的注射(农用注射)方案,用于将狐尾花叶病毒和甘蔗花叶病毒克隆接种到玉米幼苗中。以这种方式接种会导致病毒感染、病毒诱导的标记基因沉默以及 GFP 的病毒过表达。
Tags
Maize
Agroinoculation
Foxtail Mosaic Virus
Sugarcane Mosaic Virus
Viral Vectors
Recombinant
Inoculation
Coleoptile
Gene Expression
RT-PCR
GFP
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